WebCystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. WebCFTR is a well-validated targeted for development of inhibitors for therapy of secretory diarrheas and activators for therapy in cystic fibrosis. Our lab has identified and …
RCSB PDB - 5TNF: Crystal structure of the E153Q mutant of the CFTR ...
The CFTR inhibitory factor (Cif) is a protein virulence factor secreted by the Gram-negative bacteria Pseudomonas aeruginosa and Acinetobacter nosocomialis. Discovered at Dartmouth Medical School, Cif is able to alter the trafficking of select ABC transporters in eukaryotic epithelial cells, such as the cystic … See more Cif was first discovered by co-culturing P. aeruginosa with human airway epithelial cells and monitoring the resulting effect on chloride ion efflux across a polarized monolayer. After co-culture, the CFTR specific chloride ion … See more Cif belongs to the α/β hydrolase family of proteins. Its structure was determined by X-ray crystallography and consists of the canonical α/β … See more Cif is an epoxide hydrolase (EH) with unique substrate selectivity. Cif is the first example of an EH serving as a virulence factor. Based on structural comparison, it appears that the … See more WebJan 20, 2024 · 64 the CFTR Inhibitory Factor (Cif) from Pseudomonas aeruginosa [2]. 65. Cif is an epoxide hydrolase from . P. aeruginosa. that serves as a virulence factor . 66. in lung infections. Cif has been shown to decrease the concentration of the Cystic . 67. Fibrosis Transmembrane Conductance Regulator (CFTR) on the apical surface of lung . 68 healthy lunch near me cheap
Nanobody-based binding assay for the discovery of …
WebJan 29, 2010 · CFTR is the ion channel responsible for chloride secretion into the airway surface liquid (ASL) in the lung. The removal of CFTR from the cell surface leads to reduced chloride efflux, ASL dehydration, and decreased mucociliary clearance, thus facilitating the establishment of a bridgehead for bacterial infection. WebJul 1, 2015 · Pseudomonas aeruginosa secretes the CFTR inhibitory factor Cif and thus triggers loss of CFTR, an ion channel required for airway mucociliary defense. However, the mechanism of action of Cif has remained unclear. It catalyzes epoxide hydrolysis, but there is no known role for natural epoxides in CFTR regulation. It was demonstrated that the ... WebMay 30, 2024 · The CFTR inhibitory factor (Cif), which has putative orthologs in several opportunistic airway pathogens [10], is an epoxide hydrolase. Its catalytic activity … motown original name