Gapdh antibody dilution

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August undefined, 2024

WebImmunocytochemistry analysis of GAPDH in HeLa cells. Samples were incubated in GAPDH monoclonal antibody ( Product # MA1-16757) using a dilution of 1:100. … WebAll lanes : Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915) at 1/10000 dilution ... (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor ® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary ...

GAPDH Antibody Guideline: Preparation, Dilution and Applications

WebThere are 12 GAPDH antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA, IP, ICC/IF. There are 9 GAPDH antibody for … WebGlyceraldehyde-3-phosphate dehydrogenase (GAPDH), a classic glycolytic enzyme, is a multi-functional protein. It is mainly present in the cytoplasm. GAPDH is ubiquitously … h m turkey

GAPDH Antibody (0411) SCBT - Santa Cruz Biotechnology

WebPurified anti-GAPDH Antibody. Whole cell extracts (15 µg protein) from HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-GAPDH Antibody, clone FF26A/F9, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat ... WebWestern Blot: GAPDH Antibody [NB300-327] - Analysis of different cell cytosolic or nuclear enriched fractions, GAPDH antibody, dilution 1:20,000 (Red): [1] protein standard, [2] … WebNov 30, 2024 · GAPDH Antibody (0411) is an IgG1 κ mouse monoclonal GAPDH antibody that detects human GAPDH by WB, IP, IF and IHC(P). 2,990 citations. ... Rated 5 out of 5 by LHuang from Anti-GAPDH antibody Works well for western blotting at dilution of 1:1000. Date published: 2024-01-06. Rated 5 out of ... h&m turtleneck jumper

GAPDH Antibody Guideline: Preparation, Dilution and Applications

For Research Use Only GAPDH Monoclonal antibody

WebThe section was then incubated with ab8227, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody (ab6720, 1/1000 dilution) was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. WebGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis ... h&m turkey saleWebThe GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13.3 and encodes a tetramer containing identical chains. 1] The enzyme is commonly known as glycolytic enzyme and contains a binding site for NAD + (nicotinamide adenine diphosphate) and glyceraldehyde-3-phosphate. GAPDH contributes to 10-20% … hm tute bambina

"WebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产品名称. 包装. AG0122. GAPDH Rabbit Monoclonal Antibody. 50µl. AZ050. Western一抗稀释液. " - Gapdh antibody dilution

Gapdh antibody dilution

Anti-GAPDH Antibody AB2302 - EMD Millipore

WebResuspend cells in 100 µl of diluted fluorochrome-conjugated secondary antibody (prepared in Antibody Dilution Buffer at the recommended … WebMonoclonal Antibody for studying GAPDH. Cited in 5519 publications. Validated for WB, WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, GAPDH (D16H11) XP® Rabbit …

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WebWestern blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody HRP-60004 at a dilution of 1:10000. WB analysis of HeLa using HRP-60004 HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60004 (GAPDH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. Web11 rows · Anti-GAPDH, Cat. No. AB2302, is a chicken polyclonal antibody that detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is tested for use in Western …

WebGAPDH antibody used for WB at 1:1000 dilution on lysates of mouse embryonal carcinoma P19 cell line which was induced to differentiate for 5 days while being … Web(sc-32233) GAPDH Antibody (6C5) Supplier Santa Cruz Biotechnology. Host Mouse Type Primary Clonality Monoclonal (6C5) Conjugate Unconjugated Target Glyceraldehyde-3-phosphate dehydrogenase (Oryctolagus cuniculus) ... Dilutions for 3,560 citations have been found for this ...

WebRecommended dilution: WB 1:10000 - 1:760000; IHC 1:100 - 1:500; IF/ICC 1:50 - 1:200; Antibody Dilute Procedure: Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted … WebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产品 …

WebJan 6, 2024 · GAPDH Antibody (H-12) is an IgG2a κ mouse monoclonal GAPDH antibody that detects m, r, and h GAPDH by WB, IP, IF, IHC(P) and ELISA. 245 citations ... Rated 5 out of 5 by Angelika from very good antibody works on WB in dilution 1:500 in 5%BSA on mouse brain tissue. Date published: 2024-02-15. Rated 3 out of ...

WebAnti-GAPDH單株抗體 Anti-GAPDH Mouse Monoclonal Antibody (2B5) Abbkine貨號ABL1020 Anti-GAPDH Mouse Monoclonal Antibody (2B5) GAPDH單株抗體可用做loading control ... Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB(1:10000-1:100000), IHC-P … hmt watch hindi dialWebAnti-GAPDH Antibody. Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is popular affinity purified polyclonal antibody. This antibody is supported by peer reviewed publications and reliably detects Glyceraldehyde-3. Anti-GAPDH Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available … h & m tute bambinoWebThere are 12 GAPDH antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA, IP, ICC/IF. There are 9 GAPDH antibody for WB, 4 GAPDH antibody for IHC-P, 3 GAPDH antibody for ELISA, 4 GAPDH antibody for IP, 2 GAPDH antibody for ICC/IF. Among all these GAPDH antibodies, there are 3 anti … hm tuta interaWebA specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing … hmu adalahWebAnti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is popular affinity purified polyclonal antibody. This antibody is supported by peer reviewed publications and … fare gyoza hmtx industries datanyzeWebThe membrane was probed with a GAPDH polyclonal antibody (Product # PA1-987) at a dilution of 1:3000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room … h m tuscolana