Optiphase supermix
WebSep 18, 2007 · After the effector cell addition, the cells were incubated 12–16 h before being harvested, when 25 μl of cell supernatant was added to 175 μl of OptiPhase SuperMix scintillation fluid (Wallac, Loughborough, Leicestershire, U.K.) and analyzed with a MicroBeta reader (PerkinElmer, Wellesley, MA). WebJul 27, 2011 · Optiphase “SuperMix” scintillation fluid was purchased from PerkinElmer Life Sciences. α-Conotoxin MII (α-CtxMII) was obtained from Dr. J. M. McIntosh (University of Utah, Salt Lake City, UT). All other chemicals were reagent grade and, unless specified otherwise, were purchased from Sigma-Aldrich, including ascorbic acid, atropine ...
Optiphase supermix
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WebOptiphase Supermix scintillation cocktail (2.5mL) was added to each sample. Triplicate samples of 100mL aliquots of the perfusion medium were treated in the same manner as the perfused brain samples. All samples were then measured for disintegrations per minute (dpm) (1450 LSC and Luminescence Counter; Perkin Elmer, Waltham, MA, USA). WebOptiphase HiSafe 3, had been selected as a result of a previous comparative study (BLG-632, September 1991). 1.1 Experimental setup In a first stage, a limited market study was performed to establish the range and variety of different, commercially available, liquid scintillation cocktails available for purchase.
WebJan 29, 1999 · The bound IP was eluted with 1.0 M ammonium formate in 0.1 M formic acid. Fifty microliters of the eluate from each well was transferred into a Wallac 96-well plate and mixed with 200 μl of Optiphase supermix. The plate was covered with a plastic plate seal and the contents were mixed by shaking on a microtiter plate shaker for 10 min. WebNov 15, 2013 · To analyze the influence of saponins on the cell membrane-cholesterol we developed a cell culture model using ECV-304 urinary bladder carcinoma cells and …
http://www.optiphase.com/ WebJan 29, 1999 · The bound IP was eluted with 1.0 M ammonium formate in 0.1 M formic acid. Fifty microliters of the eluate from each well was transferred into a Wallac 96-well plate …
WebDec 25, 2016 · The choice of the scintillation liquid depends on the radioisotope used to label the peptide. Select the best reagent for your needs according to the instructions of …
WebMay 27, 2016 · The Optiphase™ supermix and Hionic-Fluor™ liquid scintillation cocktails and Solvable™ were purchased from Perkin Elmer (Coventry, UK). Liposomal amphotericin B (AmBisome®, Gilead Sciences, Foster City, CA, USA) and amphotericin B deoxycholate (Fungizone®, ER Squibb, Uxbridge, UK) were reconstituted according to manufacturer's … phillip collins colton nyWebPerkinElmer liquid scintillation fluid optiphase supermix solution. Liquid Scintillation Fluid Optiphase Supermix Solution, supplied by PerkinElmer, used in various techniques. Bioz … phillip collingsworth jrWeb可用作蛋白激酶抑制剂的5-氰基-4-(吡咯并[2,3b]吡啶-3-基)嘧啶衍生物专利检索，可用作蛋白激酶抑制剂的5-氰基-4-(吡咯并[2,3b]吡啶-3-基)嘧啶衍生物属于治疗内分泌系统疾病的药物专利检索，找专利汇即可免费查询专利，治疗内分泌系统疾病的药物专利汇是一家知识产权数据服务商，提供专利分析 ... try not lafeWebJan 31, 2024 · OptiPhase SuperMix ® liquid scintillation cocktail (purchased from PerkinElmer Life Sciences, Waltham, MA). 10. 30% glacial acetic acid. 11. Pierce Bradford … phillip collingsworth ohioWeb• OptiPhase SuperMix scintillation cocktail, PerkinElmer Product Code 1200-439 • Ultrapure water (17 MΩ⋅cm or equivalent) • Filter plates (Millipore MultiScreen HTS 96 well Filter Plates with glass fiber filter or equivalent, Millipore Product Code MSFB N6B 10) • Rapid filtration apparatus (Millipore 96 well plate phillip combsWebOptiPhase HiSafe Family A major technological advance in safer LSC cocktails, our OptiPhase HiSafe family of safer LSC cocktails uses the solvent di-isopropylnaphthalene … phillip colvinWebSep 9, 2010 · After 4 hours of incubation, the plates were centrifuged again at 300 g for 5 minutes and 100-μL aliquots of the supernatants from each well were transferred to a new plate containing 100 μL/well of Optiphase Supermix scintillation fluid (Perkin Elmer). Radioactivity was measured using 1450 Microbeta counter (Wallac). phillip colwell