Phosphinothricin acetyltransferase

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August undefined, 2024

WebYour search returned 12 phosphinothricin acetyltransferase (PAT) ELISA Kit across 1 supplier. Showing 1 of 1 suppliers (12 products total) > >> Select All. Select up to 5 products from below to compare or request more information. Request Info for all products Compare. WebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase (PAT) and bialaphos resistance (bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application.Following the rapid development of genome editing technology, generation of …

Transformation of Maize Cells and Regeneration of Fertile Transgenic …

WebIt codes for phosphinothricin acetyl transferase (PAT) proteins of 183 amino acids and shows 85% DNA sequence homology with another marker gene pat isolated from Streptomyces viridochromogenes. WebOct 2, 2024 · Tolerance is conferred by the enzyme phosphinothricin acetyltransferase (PAT), encoded by the pat gene from Streptomyces viridochromogenes (Herouet et al. 2005 ). This enzyme metabolizes glufosinate in transgenic plants to N -acetyl- l -glufosinate (NAG) (Müllner et al. 1993 ). soin profond visage

Identification and characterization of phosphinothricin-tripeptide ...

WebReconstitution. It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH 2 O. Storage & Stability. Stored at -20°C. After reconstitution, it is stable at … WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of … soin primary care

Resistance to glufosinate is proportional to …

Phosphinothricin - an overview ScienceDirect Topics

WebApr 11, 2024 · phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate-ammonium herbicides. DP23211 corn also contains the gene that encodes for the phosphomannose isomerase protein, which is used as a selectable marker. The petition states that DP23211 corn is unlikely to pose a plant pest risk and, therefore, should WebPhosphinothricin Resistance against PTT can be achieved by elevated levels of GS, which protect bacteria from the action of PTT: the increased activity of GSII in S. viridochromogenes may be part of the self-protection mechanism against PTT. From: Encyclopedia of Microbiology (Third Edition), 2009 Tissues View all Topics Add to Mendeley soin premier therapiste kerastaseWebNov 1, 1996 · The steps involved are catalyzed by the following enzymes: step 1, PPT N-acetyltransferase (N-ac-PPT); step 2, deaminases, including L-amino acid oxidase; step 3, transaminase. Step 4 presumably ... soin radiology department

"WebPPT N-acetyltransferase. acetyl-CoA + phosphinothricin <=> CoA + H (+) + N-acetylphosphinothricin. The substrate phosphinothricin is used as a nonselective herbicide and is a potent inhibitor of EC 6.3.1.2, a key enzyme of nitrogen metabolism in plants. All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in ... " - Phosphinothricin acetyltransferase

Phosphinothricin acetyltransferase

WebProduct: phosphinothricin acetyltransferase. confers resistance to bialophos or phosphinothricin: BlpR 373 .. 924 = 552 bp 183 amino acids = 20.6 kDa. Product: phosphinothricin acetyltransferase. confers resistance to bialophos or … Phosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + phosphinothricin CoA + N-acetylphosphinothricin

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WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from … WebOct 1, 1996 · The bar and pat genes, isolated from different Streptomyces species, both encode a phosphinothricin acetyltransferase (PAT) and are widely applied in plant …

WebApr 1, 2005 · DP23211 maize expresses an insect-specific dsRNA, an insect-active non-Bt protein, the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate … WebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector …

Webphosphinothricin-N-acetyltransferase Test. SKU: GENE-PHOS-NAT Category: Foody Safety Assays Tag: plantExtract. Related products. urinalysis test … Web2.2 Preparation of the Phosphinothricin Acetyltransferase Electrochemical Immunosensor Prior to modification, the GCE was polished with 1 μm, 0.3 µm, and 0.05 µm aluminum powder sequentially on polishing paper, with sonication in double-distilled water between each polishing step.

WebDonn G, Dirks R, Eckes P, Uijtewaal B (1990a) Transfer and expression of modified phosphinothricin-acetyltransferase gene from Streptomyces viridichromogenes in tomato, melon, carrot, and strawberry. Abstr VII Int Congr Plant Tissue Cell Cult Amsterdam: 176.

WebDec 1, 1993 · Phosphinothricin acetyltransferase activity was detected in extracts of the T0 plants. These plants were resistant to local application of the herbicide Ignite/Basta, and the resistance was inherited in T1 plants as a single dominant locus. soin profond olaplexWebGene: pat Gene Source: Streptomyces viridochromogenes Product: phosphinothricin N-acetyltransferase (PAT) enzyme Function: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation GM Trait: Glufosinate herbicide tolerance Events with gene pat soin regenerant thalacWebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … soin profondWeb2.2 Preparation of the Phosphinothricin Acetyltransferase Electrochemical Immunosensor Prior to modification, the GCE was polished with 1 μm, 0.3 µm, and 0.05 µm aluminum … slug and lettuce bookWebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... slug and lettuce blue light cardWebYour search returned 12 phosphinothricin acetyltransferase ELISA Kit across 1 supplier. Showing 1 of 1 suppliers (12 products total) > >> Select All. Select up to 5 products from … so in redditWebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) tiafenacil in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions comprising said … soin radiation oncology